ATG now offers the most sophisticated third-party service available for your Microplate reader. Our recent merger with Eastman Consulting International, brings to our customers the leading expert on servicing platereaders nationwide. Our newly developed software provides a level of sophistication in diagnostics that goes far beyond a calibration. With the ATG/Eastman diagnostics program, we can assess the full spectrum of the instrument with far greater accuracy and depth, making what we offer an “Operational Qualification”.
Photometric accuracy – Going deeper than the industry standard of 4 filters, our test reads 8 filters at 6 different wavelengths within the instrument’s operating range. We then determine the accuracy of the optical density measurements by comparing this data against the calibrated values for the plate.
- Testing those four extra filters allows us to bring the range from .25 – 1.5 OD to 0-4 OD.
- We also check the full range of the instrument’s wavelength capabilities, bringing the range from 440-635 nm up to 190-1000 nm.
Wavelength accuracy – Going beyond the industry standard of measuring3 Holmium and Didymium peaks to check the accuracy of assigned wavelengths, our tests measure 5 peaks. Using two wavelength standards in Column 11, the monochromator is swept through the instrument’s entire operating range. The data is then compared to calibrated values at several optical density peaks.
- We measure 5 Holmium peaks ranging from 360-637 nm – exceeding the standard 3 peaks range of 360-536 nm.
- We also measure 5 Didymium peaks ranging from 328-807 nm –exceeding the standard 3 peaks range of 329-681 nm.
We also test:
- Lamp intensity – Lamp output is checked through the instrument’s entire operating range at both high and low voltage. After determining the highest and lowest output wavelengths, the software checks the output at those wavelengths on all channels. This data is analyzed to determine the overall condition of the optical system.
- Electronic stability – Background noise of the optical detectors is checked by taking several readings with no light. The worst case is reported on the summary report.
- Monochromator Alignment – A steep known slope of a filter on the plate is read at multiple wavelengths and results are compared across all channels. The worst case is reported on the summary report.
- Linearity – Linearity is checked at 6 wavelengths using 8 levels of neutral density filters from blank to 4.0 OD. The slope and R^2 are compared to the calibrated values for the test plate being used.
- Flash Integrity – 10 readings are taken, each consisting of multiple flashes of the lamp. The output is compared for highest to lowest output vs. mean of outputs, allowing us to determine any weak or missing flashes.
- Kinetic air stability – “Air” channels are used to verify the optical system’s stability at 6 wavelengths as opposed to 3, while also verifying the sensitivity of the optics at 100%T.
- 384 Alignment – If an instrument is 384 well capable, this test uses columns 23 and 24 on the plate to check the plate alignment and cross talk between channels. The plate is designed with “air” positions and “dark” positions. Readings are taken and data is compared to specifications.
- Incubation chamber testing (optional) – A separate temperature plate is used to verify the accuracy and stability of the incubation chamber. The temperature plate has 5 thermocouples arranged on the plate in positions A1, A12, H1, H12 and E5. Measurements are taken every 18 seconds for 30 minutes after a 60 minute incubator warm-up. This test is for informational purposes only as there is no adjustment of temperatures available.